TISIDB

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Apical cell membrane Single-pass type I membrane protein Cell membrane Peripheral membrane protein Endosome Membrane, coated pit
Domain	PF14828 Amnionless
Function	Necessary for efficient absorption of vitamin B12 (PubMed:12590260, PubMed:14576052). Required for normal CUBN-mediated protein transport in the kidney. May direct the production of trunk mesoderm during development by modulating a bone morphogenetic protein (BMP) signaling pathway in the underlying visceral endoderm (By similarity).

> Gene Ontology [ TOP ]
Biological Process	GO:0006766 vitamin metabolic process GO:0006767 water-soluble vitamin metabolic process GO:0006898 receptor-mediated endocytosis GO:0007009 plasma membrane organization GO:0007588 excretion GO:0009235 cobalamin metabolic process GO:0015889 cobalamin transport GO:0033013 tetrapyrrole metabolic process GO:0042157 lipoprotein metabolic process GO:0043001 Golgi to plasma membrane protein transport GO:0051180 vitamin transport GO:0072657 protein localization to membrane GO:0072659 protein localization to plasma membrane GO:0090002 establishment of protein localization to plasma membrane GO:0090150 establishment of protein localization to membrane GO:1990778 protein localization to cell periphery
Molecular Function	-
Cellular Component	GO:0005905 clathrin-coated pit GO:0010008 endosome membrane GO:0016324 apical plasma membrane GO:0030139 endocytic vesicle GO:0044440 endosomal part GO:0045177 apical part of cell GO:0098589 membrane region

> KEGG and Reactome Pathway [ TOP ]
KEGG	-
Reactome	R-HSA-196741: Cobalamin (Cbl, vitamin B12) transport and metabolism R-HSA-3359462: Defective AMN causes hereditary megaloblastic anemia 1 R-HSA-3359463: Defective CUBN causes hereditary megaloblastic anemia 1 R-HSA-3296469: Defects in cobalamin (B12) metabolism R-HSA-3296482: Defects in vitamin and cofactor metabolism R-HSA-1643685: Disease R-HSA-5668914: Diseases of metabolism R-HSA-194223: HDL-mediated lipid transport R-HSA-73923: Lipid digestion, mobilization, and transport R-HSA-174824: Lipoprotein metabolism R-HSA-1430728: Metabolism R-HSA-556833: Metabolism of lipids and lipoproteins R-HSA-196854: Metabolism of vitamins and cofactors R-HSA-196849: Metabolism of water-soluble vitamins and cofactors

> KEGG and Reactome Pathway

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KEGG

Reactome

R-HSA-196741: Cobalamin (Cbl, vitamin B12) transport and metabolism
R-HSA-3359462: Defective AMN causes hereditary megaloblastic anemia 1
R-HSA-3359463: Defective CUBN causes hereditary megaloblastic anemia 1
R-HSA-3296469: Defects in cobalamin (B12) metabolism
R-HSA-3296482: Defects in vitamin and cofactor metabolism
R-HSA-1643685: Disease
R-HSA-5668914: Diseases of metabolism
R-HSA-194223: HDL-mediated lipid transport
R-HSA-73923: Lipid digestion, mobilization, and transport
R-HSA-174824: Lipoprotein metabolism
R-HSA-1430728: Metabolism
R-HSA-556833: Metabolism of lipids and lipoproteins
R-HSA-196854: Metabolism of vitamins and cofactors
R-HSA-196849: Metabolism of water-soluble vitamins and cofactors

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between AMN and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

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Statistical results of AMN in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

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Points in the above scatter plot represent the expression difference of AMN in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	0.571	0.23
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.172	0.828
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	1.12	0.0976
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	2.255	0.000523
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	1.881	0.172
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	2.729	0.104
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.38	0.629
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	1.378	0.323
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	-0.793	0.583
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	-1.011	0.546
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	-1.163	0.594
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	-0.197	0.418

> Mutation difference between responders and non-responders

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Points in the above scatter plot represent the mutation difference of AMN in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Mut/NRes	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	11.8	-11.8	0.488
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	33.3	-33.3	0.231
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	7.1	-7.1	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	3.7	0	3.7	0.27
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	3.7	0	3.7	0.314
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	4.8	0	4.8	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	7.7	0	7.7	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	2.6	0	2.6	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	4.5	0	4.5	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	0	0	0	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	0	1

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of AMN. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of AMN. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by AMN. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of AMN. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of AMN expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between AMN and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	AMN
Name	amnion associated transmembrane protein
Aliases	amnionless; amnionless homolog (mouse); PRO1028; amnionless homolog; visceral endoderm-specific type 1 trans ......
Chromosomal Location	14q32.32
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting AMN collected from DrugBank database.

> Drugs from DrugBank database

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Details on drugs targeting AMN.

ID	Name	Drug Type	Targets	#Targets
DB00200	Hydroxocobalamin	Small Molecule	AMN, CUBN, MMAA, MMAB, MMACHC, MTR, MTRR, MUT, TCN1	9

Browse AMN