TISIDB

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Basic function annotation. > Subcellular Location, Domain and Function > Gene Ontology > KEGG and Reactome Pathway

> Subcellular Location, Domain and Function [ TOP ]
Subcellular Location	Cytoplasm, cytosol
Domain	PF01128 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Function	Cytidylyltransferase required for protein O-linked mannosylation (PubMed:26687144, PubMed:27130732, PubMed:27601598, PubMed:22522420, PubMed:22522421). Catalyzes the formation of CDP-ribitol nucleotide sugar from D-ribitol 5-phosphate (PubMed:26687144, PubMed:27130732). CDP-ribitol is a substrate of FKTN during the biosynthesis of the phosphorylated O-mannosyl trisaccharide (N-acetylgalactosamine-beta-3-N-acetylglucosamine-beta-4-(phosphate-6-)mannose), a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:26687144, PubMed:27130732). Shows activity toward other pentose phosphate sugars and mediates formation of CDP-ribulose or CDP-ribose using CTP and ribulose-5-phosphate or ribose-5-phosphate, respectively (PubMed:26687144). Not Involved in dolichol production (PubMed:26687144).

> Gene Ontology [ TOP ]
Biological Process	GO:0006486 protein glycosylation GO:0006493 protein O-linked glycosylation GO:0006720 isoprenoid metabolic process GO:0007409 axonogenesis GO:0007411 axon guidance GO:0008299 isoprenoid biosynthetic process GO:0009100 glycoprotein metabolic process GO:0009101 glycoprotein biosynthetic process GO:0035268 protein mannosylation GO:0035269 protein O-linked mannosylation GO:0043413 macromolecule glycosylation GO:0048667 cell morphogenesis involved in neuron differentiation GO:0061564 axon development GO:0070085 glycosylation GO:0097485 neuron projection guidance GO:0097502 mannosylation
Molecular Function	GO:0016779 nucleotidyltransferase activity GO:0050518 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity GO:0070567 cytidylyltransferase activity
Cellular Component	-

> KEGG and Reactome Pathway [ TOP ]
KEGG	hsa00040 Pentose and glucuronate interconversions hsa01100 Metabolic pathways
Reactome	-

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Literatures that report relations between ISPD and anti-tumor immunity. The specific mechanism were also collected if the literature reports that a gene specifically promotes or inhibits the infiltration or function of T/NK cells.

> Text Mining [ TOP ]
There is no record.

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	High-throughput screening data (e.g. CRISPR-Cas9, shRNA and RNAi) for T cell-mediated killing. Genetic screen techniques can identify mechanisms of tumor cell resistance (e.g., PTPN2) and sensitivity (e.g., APLNR) to killing by cytotoxic T cells, the central effectors of anti-tumor immunity. After comprehensively searching, eight groups of screening data sets were collected in the current database. In this tab, users can check whether their selected genes cause resistance or increase sensitivity to T cell-mediated killing in various data sets.

> High-throughput Screening

[ TOP ]

Statistical results of ISPD in screening data sets for detecting immune reponses.

PMID	Screening System	Cancer Type	Cell Line	Data Set	Statistical Results	Relation to immunity
29301958	CRISPR-Cas9	melanoma	B16F10	Pmel-1 T cell	NA/NS	NA/NS
29301958	CRISPR-Cas9	melanoma	B16F10	OT-1 T cell	NA/NS	NA/NS
28783722	CRISPR-Cas9	melanoma	Mel624	2CT-CRISPR	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX+Anti-PD1	NA/NS	NA/NS
28723893	CRISPR-Cas9	melanoma	B16	GVAX	NA/NS	NA/NS
25691366	RNAi	Breast cancer	MCF7	Luc-CTL assay	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Primary screen	NA/NS	NA/NS
24476824	shRNA	melanoma	B16	Secondary screen	NA/NS	NA/NS

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Transcriptomic and genomic profiling of pre-treated tumor biopsies from responders and non-responders to immunotherapy. These data were used to identify signatures and mechanisms of response to checkpoint blockade (e.g., anti-PDL1 and anti-PD1). One example is that mutations in the gene PBRM1 benefit clinical survival of patients with clear cell renal cell carcinoma. After comprehensively searching, we collected 5 and 6 of transcriptomic and genomic data sets, respectively. In this tab, users can check whether their selected genes have significant difference of expression or mutation between responders and non-responders in various data sets. > Expression difference between responders and non-responders > Mutation difference between responders and non-responders

> Expression difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the expression difference of ISPD in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	Log2 (Fold Change)	P value
1	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	14	12	-0.085	0.72
2	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	6	5	-0.117	0.774
3	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	7	-0.072	0.841
4	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	-0.855	0.0286
5	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	-0.892	0.461
6	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	-0.822	0.494
7	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	26	23	0.105	0.725
8	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	15	11	-0.049	0.95
9	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	11	12	0.339	0.668
10	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	4	8	0.487	0.434
11	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	2	0	0	1
12	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	2	8	1.106	0.166
13	29443960	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	68	230	-0.114	0.334

> Mutation difference between responders and non-responders

[ TOP ]

Points in the above scatter plot represent the mutation difference of ISPD in various data sets.

No	PMID	Cancer type	Group	Drug	# Res	# NRes	% Mut/Res	% Diff (R vs NR)	Pval
1	25765070	Non-small cell lung cancer (NSCLC)	all	Anti-PD-1 (pembrolizumab)	14	17	0	0	1
2	25765070	Non-small cell lung cancer (NSCLC)	smoking	Anti-PD-1 (pembrolizumab)	10	3	0	0	1
3	25765070	Non-small cell lung cancer (NSCLC)	non-smoking	Anti-PD-1 (pembrolizumab)	4	14	0	0	1
4	26359337	Melanoma	all	Anti-CTLA-4 (ipilimumab)	27	73	0	0	1
5	26359337	Melanoma	BRAFi	Anti-CTLA-4 (ipilimumab)	0	14	0	0	1
6	26359337	Melanoma	non-BRAFi	Anti-CTLA-4 (ipilimumab)	27	59	0	0	1
7	26997480	Melanoma	all	Anti-PD-1 (pembrolizumab and nivolumab)	21	17	0	0	1
8	26997480	Melanoma	MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	8	6	0	0	1
9	26997480	Melanoma	non-MAPKi	Anti-PD-1 (pembrolizumab and nivolumab)	13	11	0	0	1
10	28552987	Urothelial cancer	all	Anti-PD-L1 (atezolizumab)	9	16	0	0	1
11	28552987	Urothelial cancer	smoking	Anti-PD-L1 (atezolizumab)	5	9	0	0	1
12	28552987	Urothelial cancer	non-smoking	Anti-PD-L1 (atezolizumab)	4	7	0	0	1
13	29033130	Melanoma	all	Anti-PD-1 (nivolumab)	38	27	2.6	2.6	1
14	29033130	Melanoma	NIV3-PROG	Anti-PD-1 (nivolumab)	22	13	0	0	1
15	29033130	Melanoma	NIV3-NAIVE	Anti-PD-1 (nivolumab)	16	14	6.2	6.2	1
16	29301960	Clear cell renal cell carcinoma (ccRCC)	all	Anti-PD-1 (nivolumab)	11	13	0	0	1
17	29301960	Clear cell renal cell carcinoma (ccRCC)	VEGFRi	Anti-PD-1 (nivolumab)	6	1	0	0	1
18	29301960	Clear cell renal cell carcinoma (ccRCC)	non-VEGFRi	Anti-PD-1 (nivolumab)	5	12	0	0	1

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between abundance of tumor-infiltrating lymphocytes (TILs) and expression, copy number, methylation, or mutation of ISPD. The immune-related signatures of 28 TIL types from Charoentong's study, which can be viewed in the download page. For each cancer type, the relative abundance of TILs were inferred by using gene set variation analysis (GSVA) based on gene expression profile. In this tab, users can examine which kinds of TILs might be regulated by the current gene.

> Lymphocyte [ TOP ]

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between three kinds of immunomodulators and expression, copy number, methylation, or mutation of ISPD. These immunomo-dulators were collected from Charoentong's study. In this tab, users can examine which immunomodulators might be regulated by ISPD. > Immunoinhibitor > Immunostimulator > MHC molecule

> Immunoinhibitor [ TOP ]

> Immunostimulator [ TOP ]

> MHC molecule [ TOP ]

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Relations between chemokines (or receptors) and expression, copy number, methylation, or mutation of ISPD. In this tab, users can examine which chemokines (or receptors) might be regulated by the current gene. > Chemokine > Receptor

> Chemokine [ TOP ]

> Receptor [ TOP ]

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Distribution of ISPD expression across immune and molecular subtypes. > Immune subtype > Molecular subtype

> Immune subtype [ TOP ]

> Molecular subtype [ TOP ]

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Associations between ISPD and clinical features. > Overall survival analysis > Cancer stage > Tumor grade

> Overall survival [ TOP ]

> Stage [ TOP ]

> Grade [ TOP ]

Summary
Symbol	ISPD
Name	isoprenoid synthase domain containing
Aliases	hCG_1745121; notch1-induced protein; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase homolog (Arabidopsis ......
Chromosomal Location	7p21.2
External Links	HGNC, NCBI, Ensembl, Uniprot, GeneCards
Content	Drugs targeting ISPD collected from DrugBank database.

> Drugs from DrugBank database [ TOP ]
There is no record.

Browse ISPD